Introduction: Chronic dacryocystitis is a common ophthalmologic condition characterized by inflammation of the lacrimal sac, often due to bacterial infection. Understanding the microbiological profile and antibiotic susceptibility patterns is crucial for effective treatment and prevention of complications. Materials and Methods: This study included 150 patients diagnosed with chronic dacryocystitis. Samples were collected from the lacrimal sac and subjected to microbiological analysis. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method. Inclusion criteria involved patients with persistent epiphora and discharge, while exclusion criteria included acute dacryocystitis and prior antibiotic use within one week. Results: The most common isolates were Staphylococcus aureus (35%), followed by Streptococcus pneumoniae (25%) and Pseudomonas aeruginosa (15%). Antibiotic susceptibility testing revealed high resistance to penicillin (70%) and erythromycin (60%), while gentamicin and ciprofloxacin showed the highest efficacy (90% and 85%, respectively). Ten tables summarize the microbiological and susceptibility profiles. Conclusion: The study highlights the predominance of gram-positive bacteria in chronic dacryocystitis and the rising resistance to commonly used antibiotics. Gentamicin and ciprofloxacin remain effective treatment options. These findings emphasize the need for routine microbiological evaluation and tailored antibiotic therapy.